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homework%201 - BIBC 102 Fall 2008 Homework 1 (33 pts.) 1)...

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BIBC 102 Name ___ Fall 2008 Homework 1 (33 pts.) Discussion Section_________ 1) For the reversible, first-order reaction: k 1 A B k -1 Define the following, using only starting reactant and product concentrations and rate constants. a. the net forward reaction velocity, V b. the net reverse reaction velocity, -V c. the equilibrium constant, K 2) Complete the following sentence: Enzymatic catalysis [ does/ does not ] change the equilibrium constant for a reaction, because… 3) a. Use a Lineweaver-Burke plot to determine V max and K M for the unknown enzyme that gave the following data: [S] ( μ mol/L) V 0 ( μ mol/L)/min 5 22 10 39 20 65 50 102 100 120 200 135 b. If the total enzyme concentration is 1 nmol/L, how many molecules of substrate are converted to product by one molecule of enzyme in one second? c. Calculate k cat /K M , and compare this number to those given in table 6-8 of your text.
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homework%201 - BIBC 102 Fall 2008 Homework 1 (33 pts.) 1)...

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