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proteinsLab - "Proteins BME 112 Report prepared by Lab...

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“Proteins” BME 112 Report prepared by: Lab Group:
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Abstract The goal for this lab is to determine how closely related various species of fish are. Samples of proteins from various species were inserted into an electrophoresis gel, which was allowed to run for 30 minutes. Based on the distance that the DNA fragments traveled, a conclusion was made about how closely related the fish were. After looking over the protein gel, it could be seen that the salmon and tuna were closely related, as were fish the Sea Bass and Mahi Mahi. Background The goal of this lab is to learn about gel electrophoresis. After completing the lab, students should learn how to load the gels, understand how electrophoresis works, and be able to determine the significance of the restriction fragments. The lab demonstrates a practical application of restriction enzymes and how DNA and RNA are structured. Materials & Methods Materials Needed: Electrophoresis gel Mini-protean 3 gel box TGS electrophoresis gel Pipet
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