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Unformatted text preview: Fundamental Principles: o H 3 O + + OH- 2H 2 O o Moles H 3 O + = moles OH-o Moles PC in sample = moles H 3 O + released from the column o Ion-exchange chromatography depends on the ion-exchange resin, synthetic polymer. o Cation-exchange resin has many weak acid molecules attached to the resin Procedure 1. Use the NaOH which was standardized before today 2. Prepare Column as per the instruction manual 3. Perform the column regeneration as per the instruction manual 4. Load a sample on to the regenerated column a. See the instruction manual for the instructions 5. Titrate each sample separately using a standard strong acid and strong base titration. Table of Data List of Samples Titration Volumes Total Positive charge in samples Amount of waste solution Amount of Distilled Water in step 4...
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This note was uploaded on 04/02/2008 for the course CHEM 104B taught by Professor Staff during the Spring '07 term at University of Arizona- Tucson.
- Spring '07