Study Guide for BIO 210 Lab Exam I Note: This is intended to be used as a guide for preparing for our laboratory examination. You should not consider this to be your only source for preparing for the exam, but review your lab lecture notes, re-read each exercise, and study the results, as well. Additionally, you are responsible for knowing the gram reaction and shape (morphology) of all bacterial m/o that should be listed on your microorganism list. Exercise 1--Brightfield Microscopy Know: parfocal, components of the scope, how to calculate total magnification, resolution and 4 ways to maximize resolution, proper viewing procedure, how to use immersion oil. Total magnification: ocular x objective Optimize resolution: o Raise condenser o Open diaphragm o Use blue light o Oil immersion with 100x Exercise 7 — Ubiquity of Bacteria Know: Ubiquity definition, how bacterial growth appears on/in different types of media. Ubiquity: everywhere Solid=colonies Broth=turbidity Exercise 8 -- Fungi Know: General characteristics of fungi, basic differences between molds and
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- Spring '14
- Bacteria, aseptic techniques