Lab Final Exam Review Sheet 210.doc - Study Guide for BIO 210 Lab Final 41\u2014Physiological Characteristics Know all characteristics of the tests

Lab Final Exam Review Sheet 210.doc - Study Guide for BIO...

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Study Guide for BIO 210 Lab Final Exercises 39, 40, 41—Physiological Characteristics Know : all characteristics of the tests performed in these exercises (name of test, enzyme, reagent, control microorganism, positive result, media used, additional name for some tests)—you should use the study chart from lab; IMViC reactions; be prepared to interpret results of all tests. Know the miscellaneous note information given in class for some tests. Name of test AKA Medium Enzyme Control m/o Positive result Reagent(s) Sugar fermentation n/a Phenol red broth n/a Escherichia coli Acid= yellow Gas=bubble n/a Mixed acid fermentation Methyl red test MR-VP Formic hydrogenylase Escherichia coli Red color Methyl red Butanediol fermentation Vogues- Proskauer test MR-VP n/a Enterobacter aerogenes Red color with aeration Barritt’s reagents A & B Catalase production n/a Any solid medium Catalase Staphylococcu s aureus Bubbles Hydrogen peroxide Oxidase production n/a Any solid medium Oxidase Pseudomonas aeruginosa Purple Oxidase test reagent Nitrase production n/a Nitrate broth Nitrase Escherichia coli Nitrate reagents test 1 & 2 Red color Starch hydrolysis n/a Starch agar plate Amylase Bacillus subtilis Clearing Gram’s iodine Casein hydrolysis n/a Skim milk agar plate Caseinase Bacillus subtilis Clearing n/a Lipid hydrolysis n/a Spirit blue agar plate Lipase Staphylococcu s aureus Clearing & blue precipitate n/a Tryptophan hydrolysis Indole test Tryptose broth Tryptophanase Escherichia coli Red ring Kovae’s Urea hydrolysis n/a Urea broth Urease Proteus vulgaris Neon purple n/a H 2 S production n/a SIM (sulfate, indole, motility) Cysteine disulfuriase Proteas vulgaris Black precipitant anywhere n/a Citrate production n/a Simmons citrate agar Citritase Enterobacter
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