This document is for your own personal use only and may not be redistributed in any form. Property of the University of Wisconsin Study Guide for Lectures 6, 7, 8, and 9 Microbiology 101 Spring 2018 Terms to know binary fission exponential growth planktonic growth biofilm endospore psychrophile, thermophile, mesophile pasteurization acidophile, alkaliphile halophile aerobe, microaerophile, anaerobe aerotolerant reactive oxygen species carotenoid catalase autotroph heterotroph potential energy chemotroph phototroph electron donor electron acceptor energy source oxidation reduction redox reaction electron carrier NAD + /NADH ATP substrate-level phosphorylation (SLP) ATP synthase fermentation respiration electron transport chain (ETC) quinone cytochrome flavoprotein Iron-Sulfur protein proton motive force (PMF) reaction center light harvesting center photosystem defined medium complex medium Study questions Learning goal 1. Understand the features of biofilm and planktonic growth. 1. How does planktonic growth differ from biofilm growth? 2. Where are four places you might find microbial biofilms? 3. How many generations would it take for a bacterial population to grow from one cell to a population of over 10,000 cells (or more)? 4. If the bacterium in question 1 had a 10 minute doubling time, about how long would this take (to go from 1 cell to at least 10,000 cells)? 5. What are two examples of how biofilm growth may occur in the human body?
This document is for your own personal use only and may not be redistributed in any form. Property of the University of Wisconsin Learning goal 2. Be able to describe endospore formation and understand its purpose in starvation survival. 6. What is the basic process for endospore formation and how does it differ from binary fission? 7. Which of the following pairs of terms would be better to describe the endospore formation process? a. binary fission/asymmetric cell division b. irreversible/reversible c. not reproductive/ reproductive d. phagocytosis/engulfment 8. Bacterial endospores function in Learning goal 3. Be able to explain the different environmental factors that are important in determining microbial growth. 9. How are high temperatures and low temperatures different in terms of control of microbial growth? 10. What is pasteurization? 11. Know the general classes of microbes with regard to oxygen interactions. Know what the different types of growth in the test tubes in the figure represent and be able to explain the growth patterns for each class of microbe. 8. What are reactive oxygen species, when do they occur, why are they important and what are two ways cells have to deal with them? 9. An aerotolerant microbe is best described as one that is
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