Lecture 10a Handout - Isolation and identification of viruses using laboratory animals.pdf

Applied Veterinary Virology: The isolation and identification of viruses using laboratory animals
1 | P a g e
Applied Veterinary Virology:
The isolation and identification of viruses using
laboratory animals
Authors : Prof Moritz van Vuuren
Licensed under a Creative Commons Attribution license .
TABLE OF CONTENTS
Ethics and regulations ................................................................................................................................ 3
Replacement ............................................................................................................................................ 3
Reduction ................................................................................................................................................. 4
Refinement ............................................................................................................................................... 4
Animals most commonly subjected to experimental infections or laboratory trials ........................... 4
Mice .......................................................................................................................................................... 4
Rats .......................................................................................................................................................... 5
Guinea pigs .............................................................................................................................................. 5
Rabbits ..................................................................................................................................................... 5
Experimental work with viruses ................................................................................................................ 5
Handling of specimens for viral isolation ................................................................................................. 5
Preparation of the inoculum for viral isolation ........................................................................................ 6
Routes of inoculation ................................................................................................................................. 6
Intracerebral ............................................................................................................................................. 6
Intraperitoneal .......................................................................................................................................... 6
Intramuscular ........................................................................................................................................... 7
Subcutaneous .......................................................................................................................................... 7
Intradermal ............................................................................................................................................... 7