Lecture 10a Handout - Isolation and identification of viruses using laboratory animals.pdf - Applied Veterinary Virology The isolation and

Lecture 10a Handout - Isolation and identification of viruses using laboratory animals.pdf

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Applied Veterinary Virology: The isolation and identification of viruses using laboratory animals 1 | P a g e Applied Veterinary Virology: The isolation and identification of viruses using laboratory animals Authors : Prof Moritz van Vuuren Licensed under a Creative Commons Attribution license . TABLE OF CONTENTS Ethics and regulations ................................................................................................................................ 3 Replacement ............................................................................................................................................ 3 Reduction ................................................................................................................................................. 4 Refinement ............................................................................................................................................... 4 Animals most commonly subjected to experimental infections or laboratory trials ........................... 4 Mice .......................................................................................................................................................... 4 Rats .......................................................................................................................................................... 5 Guinea pigs .............................................................................................................................................. 5 Rabbits ..................................................................................................................................................... 5 Experimental work with viruses ................................................................................................................ 5 Handling of specimens for viral isolation ................................................................................................. 5 Preparation of the inoculum for viral isolation ........................................................................................ 6 Routes of inoculation ................................................................................................................................. 6 Intracerebral ............................................................................................................................................. 6 Intraperitoneal .......................................................................................................................................... 6 Intramuscular ........................................................................................................................................... 7 Subcutaneous .......................................................................................................................................... 7 Intradermal ............................................................................................................................................... 7
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