HW 9 - Biology 202 Fall 2007 Homework 9 Due November 1st or...

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Biology 202 NAME: Gaby morales Fall 2007 T.A.: yadeeh escobedo Homework 9 Discussion Section #:9 Due: November 1 st or 2 nd 1. (2 pts) You are given a str R (streptomycin resistant) Hfr strain and an amp R (ampicillin resistant) F strain. Even when the Hfr strain is mixed with the F - strain for very long perios of time, streptomycin resistance is not transferred. State two hypotheses that explain this finding. One reason could be that they are too far apart from one another, and another reason could be that since the cells are in an environment that is constantly moving and fluid, te cytoplasm bridge can break. 2. (3 pts) With the use of P22 as a generalized transducing phage grown on a pur + pro + his + donor, a recipient strain of genotype pur pro his (defective in the synthesis of purines, proline and histidine) is infected and incubated. Afterward, transductants for pur + pro + and his + are selected individually in experiments I, II and III, respectively.
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