PR Carbohydrates_Spring2018.pdf - PR-Carbohydrate Broth(SPRING 2018 version(PR-Glucose PR-Lactose PR-Sucrose PR-Mannitol PR-Inulin or other Carbohydrate

PR Carbohydrates_Spring2018.pdf - PR-Carbohydrate...

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1 PR-Carbohydrate Broth (SPRING 2018 version) (PR-Glucose, PR-Lactose, PR-Sucrose, PR-Mannitol, PR-Inulin, or other Carbohydrate) Introduction Fermentation ability is often used to help characterize bacterial species. Bacteria are diverse in the types of carbohydrates they can ferment and diverse in the types of fermentation by-products they generate. The production of many kinds of foods and beverages relies on this diversity of fermentation processes. PR-Carbohydrate broths test fermentation ability. Each PR-Carbohydrate broth has the following ingredients: A single fermentable carbohydrate (glucose, lactose, sucrose, mannitol, inulin, or other carbohydrate) Phenol-Red pH indicator Durham Tube (a small, test tube inverted in the broth) Phenol-Red is a pH indicator that is yellow below pH 6.8, red from pH 6.8 to 7.4, and pink above pH 7.4. The broth is initially pH neutral. When a microbe is inoculated into a PR-Carb broth, that organism will either ferment the carbohydrate or not.
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  • Winter '18
  • Jamey Marsh
  • Bacteria, Durham tube

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