Bile Esculin_Spring2018.pdf - Bile Esculin(Spring 2018 version Introduction Bile Esculin is a selective and differential medium It contains bile(which

Bile Esculin_Spring2018.pdf - Bile Esculin(Spring 2018...

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Bile Esculin (Spring 2018 version) Introduction Bile Esculin is a selective and differential medium. It contains bile (which inhibits many Gram-positive organisms including staphylococci). It contains esculin, glucose, and peptone as nutrient sources and ferric citrate as a color indicator. Esculin is a carbohydrate linked to an alcohol. Many organisms can hydrolyze esculin but very few can do so in the presence of bile. Organisms that do metabolize esculin produce esculetin as a by-product. Esculetin reacts with ferric citrate to form a dark (black) coloration. Results + = Positive (Any blackening of the agar)
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  • Winter '18
  • Jamey Marsh
  • Bacteria, bile esculin, esculin

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