chromatographylab - Week of CH116-J1 Chromatography 1 The...

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Week of October 20, 2008 CH116-J1 Chromatography 1. The TLC Experiments . 2-propanol acetic acid 15:2 H 2 O 1 propanol: acetone 1:1:1 water distance color R f value distance color R f value distance color R f value Solvent 7 7 7.5 Basic fuchsia 4.8 fuchsia 0.714 5.5 fuchsia 0.786 2.2 fuchsia 0.293 Fluorescein 6.9 yellow 0.986 5.6 yellow 0.800 2.5 yellow 0.333 Methyl red 4.1 pink 0.586 5.2 pink 0.743 0.5 2 spots, orange and pink 0.067 New methylene blue 2.5 blue 0.357 5.2 blue 0.743 0.3 blue 0.04 Bromocresol green 5.5 olive 0.786 5.6 green 0.800 2.9 green 0.387 Unknown 718 5 for fuchsia, 1.5 for yellow 2 spots, yellow and fuchsia 0.714 for fuchsia 0.214 for yellow 5.5 for fuchsia, 5.3 for yellow 2 spots, yellow and fuchsia 0.786 for yellow 0.747 for fuchsia 1.1 fuchsia 0.147 2. The Class Data. water 2 propanol acetic acid 15:2 H 2 O,acetone 1-propanol acetic acid 1:1:1:1 H 2 O 1-propanol: acetone 1:1:1 R f value R f value R f value R f value Basic fuchsia 1 0.1143 0.4474 0.9250 0.8816 2 0.1818 0.6842 0.8679 0.786 3 0.75 0.67 0.87 Fluorecein
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1 0.0714 0.9474 0.9730 0.9079 2 0.2984 0.8684 0.9623 0.800 3 1.0 0.76 0.82 Methyl red 1 0.0286 0.6053 0.9250 0.9352 2 0.039 0.6053 0.8679 0.743 3 1.03 0.57 0.95 New methylene blue 1 0.00 0.8684 0.8750 0.7237 2 0 0.2632 0.7736 0.743 3 0.28 0.26 0.02 Bromocresol green 1 0.185 0.8421
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