lecture 10

lecture 10 - Lecture 10 Molecular Markers I Repeated...

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Lecture 10 Molecular Markers I Repeated sequences, VNTRs, microsatellites, DNA fingerprinting. Griffiths et al. 9 th edition; Parts of Chapters 1, 4, 14 and 20. Published papers and books.
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Is all this DNA created equal?
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Why is our genome not the biggest one? Organisms Relative size Bacteria 0.0014 1/690 Yeast 0.004 1/265 Fruit fly 0.06 1/18 Fugu (Fish) 0.120 1/8 Human 1 Mouse 1 Salamander 20
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The C -value paradox: The lack of correlation between genome size and the biological complexity of an organism. The C -value: The DNA content of an organism
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Classes of eukaryotic DNA sequences 1. Unique or single copy DNA sequences (1-10 copies / genome ; 40-70% ; average 800-1200bp; most of structural genes) 2. Moderately repetitive DNA sequences (10-100,000 copies / genome ; average 300 bp; genes for rRNA and ribosomal proteins; transposons) 3. Highly repetitive DNA sequences (more than 100,000 copies / genome ; satellite and non-satellite DNAs)
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In prokaryotes, centrifugation of fragmented DNA on a CsCl gradient produces a single band at a given density , irrespective of the size of the fragments. (DNA density will increase with increasing percentage of G:C) In eukaryotes, the same experiment lead to the visualization of a large main band (unique copy DNA) and one or more small bands ( satellite bands) containing the satellite DNA. But where are they located?
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This note was uploaded on 08/08/2009 for the course BIOL 202 taught by Professor Y during the Spring '09 term at McGill.

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lecture 10 - Lecture 10 Molecular Markers I Repeated...

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