lecture 11

lecture 11 - Lecture 11 Molecular Markers II RFLPs, SNPs,...

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Lecture 11 Molecular Markers II RFLPs, SNPs, ASOs and the use of PCR. Griffiths et al. 9 th edition; Parts of Chapters 2, 3, 11, 12, and 14. Published papers and books.
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Thus digested DNA fragments can be separated and identified Fig 20-11
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The different restriction fragments of a linear molecule can also be ordered relative to others. However, the restriction enzyme will not cut the DNA if its restriction site is mutated, moreover mutations can create new restriction sites: How similar is our DNA ? Restriction Fragment-Length Polymorphism RFLP
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Fig. 2-20 RFLP A/A a/a A/a a/A
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Detection of other mutations by RFLP Deletion removes a site Insertion creates a site
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Pedigree analysis: a reminder Fig. 2-28
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RFLP: Distinguishing 2 DNA molecules P 32 -dCTP P 32 -dCTP Fig 12-10 (8 th edition) Fig. 4-15
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Fig 12-10 (8 th edition) Fig. 4-15 Interpret results
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could be analysed at the protein level … Fig. 6-5 Fig. 6-6 Sickle-cell anemia is due to a mutation (single amino acid substitution) in the hemoglobin ( Hb) gene. Sickled cell
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This note was uploaded on 08/08/2009 for the course BIOL 202 taught by Professor Y during the Spring '09 term at McGill.

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lecture 11 - Lecture 11 Molecular Markers II RFLPs, SNPs,...

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