lecture 12 - 13

lecture 12 - 13 - Lectures 12 and 13 Gene cloning : the...

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Lectures 12 and 13 Gene cloning : the tools needed and how to use them Griffiths et al.; Parts of Chapter 20. Published papers and books.
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An overview: Fig 11-1 (8 th edition)
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A vector is a molecule that can contain a piece of foreign DNA. Ideally: - It should be self-replicating , and thus being able to self- propagation (contains an origin of replication). - It should be able to accept foreign molecules (presence of restriction sites = or ≠ cloning site). - It should contain selectable markers (Amp and Tet). Fig 11-7 (8 th edition) Fig. 20-5
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CLONING: Generating recombinant (hybrid) DNA molecules Fig 20-2 vector (Fragment of ) Gene of interest
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In more details: Fig 20-3
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Cloning: how amplification works… Fig. 20-4
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… the bacteria is doing all the job! Fig. 20-4
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Fig. 20.5 ?? Or BamH1 LacZ Lac insert Z X-Gal Blue White (Up to 10 kb) Selecting plasmids with DNA insertion With a multiple cloning site
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Clones containing DNA fragment will form colonies Fig. 5-3
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.. But 10 kb is a relatively
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This note was uploaded on 08/08/2009 for the course BIOL 202 taught by Professor Y during the Spring '09 term at McGill.

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lecture 12 - 13 - Lectures 12 and 13 Gene cloning : the...

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