2000Final

2000Final - Bio115, Winter 2000 Final Exam, March 16 Name:_...

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Bio115, Winter 2000 Name:_______________________ Final Exam, March 16 1 Instructions: Write your name in ink at the top of every page. Do not write on the back of pages. Restrict your answers to the space provided. Excessive or ambiguous answers will count against you when assigning partial credit. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Answer the following questions with "true" or "false". Provide your reasoning for each "false" answer. Correct "true" answers are worth one point. Correct "false" answers with appropriate reasoning are worth 3 points. 1. The energy that DNA helicases use to drive the unwinding of DNA at replication forks is derived from the charge-charge interactions formed between RPA and newly single-stranded DNA. 2. One theory of the origin of introns posits that they were originally present in bacteria, but by putting a premium on rapid DNA replication, they were selected against and eventually lost. 3. Chain terminating nucleotides prevent further replication of DNA because they lack 5’ phosphates. 4. A major source of DNA damage is due to spontaneous depurination or deamination of nucleotides. This type of damage is typically repaired via the base-excision-repair pathway.
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Bio115, Winter 2000 Name:_______________________ Final Exam, March 16 2 5. In the nucleotide excision repair pathway, a patch of damaged DNA is removed from a chromosome and replaced by a normal DNA sequence from the homologous chromosome by gene conversion. 6. In eukaryotes, one unresolved question about the mismatch repair pathway is how the cell decides which DNA strand is mutant and which is wild-type. 7. Exons are typically used as probes for zoo blots because they are smaller than introns and therefore easier to manipulate. 8. If, in a Mat a cell, the HML alpha locus is replaced with HMR a , then that cell will not give rise to an alpha cell for two cell divisions. 9. When comparing homologous genes between closely related organisms, you will typically find that the size of introns is variable, but that their organization (i.e. with respect to the intron-exon junctions) is constant. 10. The same evolutionary processes that lead to production of gene families may also lead to duplication and spreading of exons throughout the genome.
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Bio115, Winter 2000 Name:_______________________ Final Exam, March 16 3 11. DNA replication and gene transcription are similar in that, in both processes, the mostly highly regulated step is the first, the initiation step. 12. In a cDNA library, every gene in the genome is represented. In a genomic DNA library, only a fraction of genes are represented. 13. Poly-linkers are flexible hinge regions of proteins that separate domains of protein function encoded by different exons. 14.
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2000Final - Bio115, Winter 2000 Final Exam, March 16 Name:_...

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