2000MT1

2000MT1 - Bio 115 Winter 2000 Midterm#1 January 25 Name...

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Bio 115, Winter 2000 Name__________________ Midterm #1, January 25 ________________________________________________________________________ page 1 page total: Instructions: Write your name in ink at the top of every page. Do not write on the back of pages. Restrict your answers to the space provided. Excessive or ambiguous answers will count against you when assigning partial credit. Remember the lesson of the boiled Buddhist: The human mind can overcome all adversity. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Answer the following questions with "true" or "false". Provide your reasoning for each "false" answer. Correct "true" answers are worth one point. Correct "false" answers with appropriate reasoning are worth 3 points. 1. Agarose gel electrophoresis is often used to separate proteins based upon their size. 2. The helicase activity of TFIIH is used to unwind DNA strands throughout the process of transcription elongation until transcription termination signals are reached. 3. RNA polymerase II can be differentiated from other RNA polymerases by the repeats at the carboxy-terminus of its large subunit. 4. Most DNA binding proteins bind in the minor groove of DNA. This is because the tight spaces of the minor groove allow for very tight and specific protein-DNA interactions. 5. The feature of Taq polymerase that makes it ideal for use in PCR is its high rate of polymerization of DNA.
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Bio 115, Winter 2000 Name__________________ Midterm #1, January 25 ________________________________________________________________________ page 2 page total: 6. Except at TATA less promoters, TBP is required for all nuclear transcription in eukaryotes. 7. Subunits of E. coli RNA polymerase share homology with several subunits of human RNA polymerase I. 8. A major way in which DNA binding proteins distinguish between specific DNA sequences involves contacts between the protein and the phosphate backbone of DNA. 9. A limitation of gel mobility shift assays is that binding of more that one protein to the probe, if it occurs, cannot be detected. 10. It is not essential that probes for gel mobility shift experiments be labeled at only one end. 11.
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This document was uploaded on 08/12/2009.

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2000MT1 - Bio 115 Winter 2000 Midterm#1 January 25 Name...

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