2000MT1answers

2000MT1answers - Bio 115, Winter 2000 Midterm #1, January...

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Bio 115, Winter 2000 Name__________________ Midterm #1, January 25 ________________________________________________________________________ page 1 page total: Instructions: Write your name in ink at the top of every page. Do not write on the back of pages. Restrict your answers to the space provided. Excessive or ambiguous answers will count against you when assigning partial credit. Remember the lesson of the boiled Buddhist: The human mind can overcome all adversity. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Answer the following questions with "true" or "false". Provide your reasoning for each "false" answer. Correct "true" answers are worth one point. Correct "false" answers with appropriate reasoning are worth 3 points. (1pt for "false", 2 for reasoning) 1. Agarose gel electrophoresis is often used to separate proteins based upon their size. False- Agarose electrophoresis is used to separate DNA by size. Proteins are separated by size on SDS-polyacrylamide gels. Although proteins can be run on agarose gels, they may not separate according to size on these gels, because in the absence of SDS proteins will fold up in to different shapes resulting in electrophoretic mobilities not directly related to their molecular weight. 2. The helicase activity of TFIIH is used to unwind DNA strands throughout the process of transcription elongation until transcription termination signals are reached. False- TFIIH is required for initiation not elongation. It is not associated with elongating polymerase. 3. RNA polymerase II can be differentiated from other RNA polymerases by the repeats at the carboxy-terminus of its large subunit. True- This refers to the CTD, which is unique to Pol II. 4. Most DNA binding proteins bind in the minor groove of DNA. This is because the tight spaces of the minor groove allow for very tight and specific protein-DNA interactions. False- Most DNA binding proteins bind in the major groove. They have more room to fit into the DNA, and more base specific information, which allows discrimination between different DNA sequences is available. 5. The feature of Taq polymerase that makes it ideal for use in PCR is its high rate of polymerization of DNA. False- The feature of Taq polymerase that makes it useful for PCR is its ability to go through multiple cycles of heating and cooling without becoming inactivated or denatured.
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Bio 115, Winter 2000 Name__________________ Midterm #1, January 25 ________________________________________________________________________ page 2 page total: 6. Except at TATA less promoters, TBP is required for all nuclear transcription in eukaryotes. False-TBP is required at all nuclear promoters, regardless of the presence or absence of the TATA box. 7.
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2000MT1answers - Bio 115, Winter 2000 Midterm #1, January...

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