MT1_2001 - Bio 115 Winter 2001 Midterm#1 January 30 Name...

Info icon This preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Bio 115, Winter 2001 Name__________________ Midterm #1, January 30 ________________________________________________________________________ page 1 page total: Instructions: Write your name in ink at the top of every page. Do not write on the back of pages. Restrict your answers to the space provided. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Answer the following questions with "true" or "false". Provide your reasoning for each "false" answer. Correct "true" answers are worth 1 point. Correct "false" answers with appropriate reasoning are worth 3 points. 1. Squelching refers to a mechanism that cells normally use to repress transcription of specific genes. 2. Mediator complex was identified by virtue of its ability to overcome squelching when added to in vitro transcription reactions. 3. Mutations in genes encoding subunits of the mediator complex can cause defects identical to those caused by loss of particular sequence specific transcriptional activators. 4. One potential way that transcriptional activators can regulate the rate of transcription initiation is to alter TFIIH activity.
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Bio 115, Winter 2001 Name__________________ Midterm #1, January 30 ________________________________________________________________________ page 2 page total: 5. Restriction enzymes bind to specific DNA sequences. 6. No single cDNA library contains copies of every protein-encoding gene in an organism’s genome. 7. When constructing a cDNA library from yeast cells, a YAC is the most appropriate vector. 8. The key breakthrough that has made PCR a viable technology is the identification of thermostable DNA ligase. 9. In general, the average size of a eukaryotic organism’s genes is correlated to the organism’s evolutionary complexity. This is because complex eukaryotes have larger proteins, which necessitates larger genes. 10. Strategies that eukaryotes use for alternative gene expression include use of alternative promoters, alternative splice sites and alternative polyadenylation sites. 11. In addition to accepting larger inserts than phage vectors, cosmids are easier to work with, and their DNA is easier to isolate.
Image of page 2
Bio 115, Winter 2001 Name__________________ Midterm #1, January 30 ________________________________________________________________________ page 3 page total: 12. Cosmids are the ideal vectors with which to construct cDNA expression libraries. 13. One theory of the origin of splicing holds that splicing increases the rate of evolution by encouraging unequal crossover events.
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern