MT1_2001

MT1_2001 - Bio 115 Winter 2001 Midterm#1 January 30 Name...

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Bio 115, Winter 2001 Name__________________ Midterm #1, January 30 ________________________________________________________________________ page 1 page total: Instructions: Write your name in ink at the top of every page. Do not write on the back of pages. Restrict your answers to the space provided. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Answer the following questions with "true" or "false". Provide your reasoning for each "false" answer. Correct "true" answers are worth 1 point. Correct "false" answers with appropriate reasoning are worth 3 points. 1. Squelching refers to a mechanism that cells normally use to repress transcription of specific genes. 2. Mediator complex was identified by virtue of its ability to overcome squelching when added to in vitro transcription reactions. 3. Mutations in genes encoding subunits of the mediator complex can cause defects identical to those caused by loss of particular sequence specific transcriptional activators. 4. One potential way that transcriptional activators can regulate the rate of transcription initiation is to alter TFIIH activity.
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Bio 115, Winter 2001 Name__________________ Midterm #1, January 30 ________________________________________________________________________ page 2 page total: 5. Restriction enzymes bind to specific DNA sequences. 6. No single cDNA library contains copies of every protein-encoding gene in an organism’s genome. 7. When constructing a cDNA library from yeast cells, a YAC is the most appropriate vector. 8. The key breakthrough that has made PCR a viable technology is the identification of thermostable DNA ligase. 9. In general, the average size of a eukaryotic organism’s genes is correlated to the organism’s evolutionary complexity. This is because complex eukaryotes have larger proteins, which necessitates larger genes. 10. Strategies that eukaryotes use for alternative gene expression include use of alternative promoters, alternative splice sites and alternative polyadenylation sites. 11. In addition to accepting larger inserts than phage vectors, cosmids are easier to work with, and their DNA is easier to isolate.
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Bio 115, Winter 2001 Name__________________ Midterm #1, January 30 ________________________________________________________________________ page 3 page total: 12. Cosmids are the ideal vectors with which to construct cDNA expression libraries. 13. One theory of the origin of splicing holds that splicing increases the rate of evolution by encouraging unequal crossover events.
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This document was uploaded on 08/12/2009.

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MT1_2001 - Bio 115 Winter 2001 Midterm#1 January 30 Name...

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