MT2_2001-key - Bio 115, Winter 2001 Midterm #2, February 22...

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Bio 115, Winter 2001 Name__________________ Midterm #2, February 22 ________________________________________________________________________ page 1 page total: Instructions: Write your name in ink at the top of every page. Do not write on the back of pages. Restrict your answers to the space provided. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Answer the following questions with "true" or "false". Provide your reasoning for each "false" answer. Correct "true" answers are worth 1 point. Correct "false" answers with appropriate reasoning are worth 3 points. 1. The nucleolus is the subcompartment of the nucleus that contains all of the heterochromatin. False- The nucleolus is the subcompartment of the nucleus in which rRNA is transcribed. (also site of ribosome assembly and assembly of other structural RNA-protein complexes) 2. In most eukaryotes, rDNA genes are arranged in arrays of hundreds or thousands of tandemly repeated genes. True 3. The specificity of RNA Pol I for rDNA genes is dependent upon the ability of UBF to directly bind and recruit Pol I to the promoter. False-UBF1 does not directly bind and recruit Pol I. That is the job of one of the TAF I s that associates with SL-1. 4. Transcription pre-initiation complexes on Pol I and Pol III transcribed genes are much more stable than those that assemble on Pol II transcribed genes. True 5. One of the subunits of TFIIIB shares sequence and functional similarities with TFIIB. True
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Bio 115, Winter 2001 Name__________________ Midterm #2, February 22 ________________________________________________________________________ page 2 page total: 6. The most appropriate way to identify heterochromatin is to stain nuclei with the Fuelgen stain. True 7. Karyotypic analysis can be used to determine if specific genes are in chromatin structures that allow their transcription. False- Karyotypic analysis is used to determine the number and gross composition of chromosomes, the karyotype, in an individuals cells/genome. 8. By purifying nuclei, performing micrococcal nuclease digestion of the chromatin, removing all proteins (including histones), running the purified DNA on a gel and staining, it was determined that most of the DNA in eukaryotic nuclei must be assembled into regularly spaced nucleosomes. True 9. At the end of an indirect endlabeling experiment, chromatin is analyzed by western blotting. False-At the end on an indirect endlabeling experiment, DNA is purified from chromatin and analyzed by Southern blotting. 10. To allow efficient transcription elongation by RNA polymerase II, the nucleosomes that cover a gene must be permanently removed. False- Highly transcribed genes are still associated with nucleosomes, deomonstrating that whatever activity overcomes the nucleosomal block to elongation does not permanently remove nucleosomes from the gene. (There is no evidence that this activity is in any way related to CAF1 or DNA replication.) 11. Phosphodiester exchange, the key chemical event in splicing of group I, group II and pre-
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MT2_2001-key - Bio 115, Winter 2001 Midterm #2, February 22...

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