sample3Finalkey

sample3Finalkey - Bio 115, Final Exam Name_ Instructions:...

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Bio 115, Final Exam Name________________________________ ________________________________________________________________________ page 1 page total: Instructions: Write your name in ink at the top of every page. Do not write on the back of pages. Restrict your answers to the space provided. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ 1. a. Diagram the central dogma of molecular biology. (2 pts.) DNA =>RNA=>Protein b. Label the two straight arrows. (2 pts.) Transcription and translation c. Give two examples of exceptions to the central dogma. (2 pts.) alternative splicing (more than one expression product / gene) reverse transcription – can go from RNA to DNA catalytic RNA- don’t need to go to protein for enzymatic activity etc. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ 2. a. Diagram a typical eukaryotic Pol II gene (DNA) sitting in the genome: Designate the following features: transcription initiation site, core promoter, terminator, enhancer elements, polyadenylation site, three exons, two introns, upstream regulatory element, transcription unit, untranslated regions, (4 pts.) b. Diagram the unprocessed pre-mRNA transcript that would correspond to this gene. (2 pts.) c. The second exon can be skipped by alternative splicing. Diagram the possible mRNA products of this gene after RNA processing. (3 pts.)
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Bio 115, Final Exam Name________________________________ ________________________________________________________________________ page 2 page total: Answer the following questions with "true" or "false". (3 points each) For each "true" answer, give an example or explanation to support the statement. For each "false" answer, explain why the statement is false. 3. Guanosine serves as the nucleophile in the first step of pre-mRNA splicing. False, guanosine serves as the first step nucleophile for Group I intron splicing or The branchpoint adneosine serves as the first step nucleophile for pre-mRNA splicing 4. Transcription factors recognize specific DNA sequences by making hydrogen bonds with the phosphate backbone. False, Transcription factors recognize specific DNA sequences by making hydrogen bonds with the bases (A,C,G,T) 5. Microarray analysis can only indirectly compare protein expression profiles between two experimental conditions. True, microarrays measure mRNA levels directly. (Protein expression can be estimated from that.) 6. Shotgun sequencing strategies rely on sequencing a given chromosomal region more than one time. True, one needs overlapping sequences to assemble the contiguous genomic sequence. (or a diagram of this) 7. A single “jumping” event of a transposon can affect more than one gene. True, the transoposon can disrupt both the donor and target gene.
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sample3Finalkey - Bio 115, Final Exam Name_ Instructions:...

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