sample3MT1

sample3MT1 - Bio 115, Spring 2005 Midterm #1, April 21...

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Bio 115, Spring 2005 Name________________________________ Midterm #1, April 21 ________________________________________________________________________ page 1 page total: Instructions: Write your name in ink at the top of every page. Do not write on the back of pages. Restrict your answers to the space provided. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ 1. Decipher the password “cdogdrp” (You can either spell it out or draw it out.) (1 point) +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Answer the following questions with "true" or "false". For each "true" answer, give an example or explanation that supports the statement. For each "false" answer, explain why the statement is false. (3 points each) 2. A cDNA library of full-length clones can potentially contain more unique (i.e. different) sequences than the number of genes in the organism from which it was derived. 3. The transcription initiation site of a gene is always found downstream of the promoter. 4. The size of an organism’s genome is not directly proportional to the number of genes encoded. 5. A characteristic of eukaryotic genomes is the absence of polycistronic gene transcripts. 6. The CTD stands for “cytoplasmic tail domain” of Pol I. 7. Purified RNA polymerase II does not bind the core promoter efficiently. 8. The polyadenylation site for a RNA Pol II transcript is specified by a protein-RNA interaction. 9. The orientation of an enhancer element is important for transcriptional activation.
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Bio 115, Spring 2005 Name________________________________ Midterm #1, April 21 ________________________________________________________________________ page 2 page total: +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Short answer questions. Limit your answer to the space provided. (4 points each) 10. The restriction enzyme Apa I recognizes “GGGCCC” and cuts to leave 4 nt 5’ overhangs. Draw the two double stranded ends that result from this cleavage. 11. Why is regulation of RNA Pol II transcribed genes so much more complex than that genes transcribed by RNA polymerases I and III? 12. Why can’t you use a western blot to identify a protein that has not been previously isolated? 13. Why is it important to separate the processes of transcription and translation in eukaryotes into different cell compartments? 14.
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sample3MT1 - Bio 115, Spring 2005 Midterm #1, April 21...

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