sample3MT1key - Bio 115 Midterm #1 Name_ Instructions:...

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Bio 115 Name________________________________ Midterm #1 ________________________________________________________________________ page 1 page total: Instructions: Write your name in ink at the top of every page. Do not write on the back of pages. Restrict your answers to the space provided. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ 1. Decipher the password “cdogdrp” (You can either spell it out or draw it out.) (1 point) Central Dog ma: D NA=>R NA=>P rotein +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Answer the following questions with "true" or "false". For each "true" answer, give an example or explanation that supports the statement. For each "false" answer, explain why the statement is false. (3 points each) 2. The size of an organism’s genome is not directly proportional to the number of genes encoded. True. Eukaryotic genomes can have many more times number of basepairs of DNA than prokaryotic genomes, but only a few fold more genes. (Compare the ratio human genome size and gene number to that of E. coli or yeast). 3. A cDNA library of full-length clones can potentially contain more unique (i.e. different) sequences than the number of genes in the organism from which it was derived. True. Alternative splicing can theoretically generate many different transcripts from a single gene. 4. The transcription initiation site of a gene is always found downstream of the promoter. False. Promoters for PolIII genes can be located in the transcription unit. In those cases the initation site is upstream of the promoter 5. A characteristic of eukaryotic genomes is the absence of polycistronic gene transcripts. False. The rRNA gene contains information for three transcripts. 6. Purified RNA polymerase II does not bind the core promoter efficiently. True. PolII requires the General Transcription Factors to bind the core promoter. 7. The CTD stands for “cytoplasmic tail domain” of Pol I. False. CTD stands for “C-terminal domain” of PolII. 8. The 3’ end cleavage site for a RNA Pol II transcript is specified by a protein-RNA interaction. True. This is the polyadenylation seuqence recognized by the polyadenylation complex. 9. The orientation of an enhancer element is important for transcriptional activation. False. The spacing and orientation enhancers do not affect their ability to activate transcription.
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Bio 115 Name________________________________ Midterm #1 ________________________________________________________________________ page 2 page total: +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Short answer questions. Limit your answer to the space provided.
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sample3MT1key - Bio 115 Midterm #1 Name_ Instructions:...

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