BIOL125_Lab Manual

BIOL125_Lab Manual - Sairam Microbiology BIOL125L...

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Sairam Microbiology BIOL125L Laboratory Manual FALL08 – SPRING 09 http://activity.ntsec.gov.tw/lifeworld/english/content/images/en_dis_c2a.jpg
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2 TABLE OF CONTENTS Experiment Topic Page 1 Laboratory Safety 4 2 Ubiquity 5 3 Growth Characteristics of Microorganisms 9 4 Microscope 14 5 Aseptic Transfer 20 6 Streak Plate method: Isolating a pure culture 24 7 Smear preparation and Simple Staining method 27 8 Gram Staining method 30 9 Capsule Staining method 32 10 Acid Fast Staining method 34 11 Endospore Staining method 36 12 Pipet Usage 38 13 Serial dilution and spread plate method: Isolating a pure culture and measuring bacterial Concentration 41 14 Spectrophotometry: Measuring Bacterial Concentration 44 15 Bacterial Growth Curve 48 A1 Instructions for Unknown bacterium Identification 50 16 Morphological characteristics determination of unknown bacterium 54 17 Optimum Temperature Requirement/Broth characteristics 55 18 Motility 56 A2 Metabolism Overview 58 19 Oxidase 62 20 Catalase 63 21 Oxygen Requirements 64 22 Carbohydrate Fermentation 67 23 Citrate Utilization 69 24 Biooxidations 70 25 Hydrolytic enzymes 75 26 Amino Acid Utilization 79 27 Mannitol utilization 82 28 Litmus milk test 83 A3 Dichotomous key for unknown genus identification 85 A4 Guide lines for writing unknown paper 88 29 Thermal Death 93 30 Disinfectants and Antiseptics 95 31 Antibiotics 97 32 Dental Caries 100 33 Micropipet Usage 102 34 Transformation 104 35 Plasmid Isolation 107
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3 36 Agarose Gel Electrophoresis 109 37 ELISA 111 38 Water Analysis using Biochemical Tests 113 39 Water Analysis using PCR 116 40 Enterobacteriaceae Identification 119 41 Effectiveness of Hand washing and Scrubbing 122 42 Koch’s Hypothesis 124 43 Protista 126 44 Fungi 128 45 Parasitic worms 130 46 Arthropod Vectors 132 MESSAGE TO THE STUDENT Each laboratory is designed in a way that it explains one or more concepts learned in each of the corresponding lecture. Preparation for lab before coming to class is as essential as preparing ahead of time for the lecture. You will be using lecture handouts and the lab manual as reference material. The guidelines to be followed before each lab are as follows: Before coming to class the following should be written : ! Purpose of the Lab, ! Materials used in the experiment ! Procedure : Draw the flow chart of the procedure described and get it signed by the instructor at the beginning of every lab After experiment is performed, the following should be written : ! Results should include the following where necessary: o Draw and label the observed specimen o Record the data in a table and compare the results o Record the data and draw a graph o Record the data calculate ! Conclusions : Write the summary of the results and draw conclusions ! Complete the post lab exercises. Submit work of those experiments as instructed by the instructor, on the day of Lab
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BIOL125_Lab Manual - Sairam Microbiology BIOL125L...

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