BIOL125_Lab Workbook

BIOL125_Lab Workbook - 1 Jai Sairam Microbiology BIOL125L...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: 1 Jai Sairam Microbiology BIOL125L Laboratory Work Book FALL08 SPRING 09 http://activity.ntsec.gov.tw/lifeworld/english/content/images/en_dis_c2a.jpg 2 TABLE OF CONTENTS Experiment Topic Page 1 Laboratory Safety 4 2 Ubiquity 5 3 Growth Characteristics of Microorganisms 8 4 Microscope 12 5 Aseptic Transfer 18 6 Streak Plate method: Isolating a pure culture 21 7 Smear and Simple Staining method 24 8 Gram Staining method 27 9 Capsule Staining method 31 10 Acid Fast Staining method 34 11 Endospore Staining method 37 12 Pipet Usage 40 13 Serial dilution and spread plate method: Isolating a pure culture and measuring Bacterial Concentration 42 14 Spectrophotometry: Measuring Bacterial Concentration 45 15 Bacterial Growth Curve 49 16 Morphological characteristics determination of unknown bacterium 53 17 Broth Characteristics/Optimum Temperature Requirement 55 18 Motility 58 19 Oxidase 60 20 Catalase 62 21 Oxygen Requirements 64 22 Carbohydrate Fermentation 67 23 Citrate Utilization 69 24 Biooxidations 71 25 Hydrolytic enzymes 74 26 Amino Acid Utilization 77 27 Mannitol utilization 80 28 Litmus milk test 82 A1 Table for all tests (General Log sheet) 84 A2 Table for specific tests 87 A3 Descriptive Chart of the unknown 88 A4 Dichotomous key for unknown 89 29 Thermal Death 90 30 Disinfectants and Antiseptics 95 31 Antibiotics 98 32 Dental Caries 101 33 Micropipet Usage 104 34 Transformation 105 35 Plasmid Isolation 108 3 36 Agarose Gel Electrophoresis 110 37 ELISA 114 38 Water Analysis using Biochemical Tests 117 39 Water Analysis using PCR 121 40 Enterobacteriaceae Identification 125 41 Effectiveness of Hand washing and Scrubbing 128 42 Kochs Hypothesis 131 43 Protista 135 44 Fungi 143 45 Parasitic worms 148 46 Arthropod Vectors 155 MESSAGE TO THE STUDENT Each laboratory is designed in a way that it explains one or more concepts learned in each of the corresponding lecture. Preparation for lab before coming to class is as essential as preparing ahead of time for the lecture. You will be using lecture handouts and the lab manual as reference material. The guidelines to be followed before each lab are as follows: Before coming to class the following should be written : ! Purpose of the Lab, ! Materials used in the experiment, ! Procedure : Draw the flow chart of the procedure described and get it signed by the instructor at the beginning of every lab After experiment is performed, the following should be written : ! Results should include the following where necessary: o Draw and label the observed specimen o Record the data in a table and compare the results o Record the data and draw a graph o Record the data calculate ! Conclusions : Write the summary of the results and draw conclusions ! Complete the post lab exercises....
View Full Document

Page1 / 162

BIOL125_Lab Workbook - 1 Jai Sairam Microbiology BIOL125L...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online