Capping & Polyadeynyl - Multiple Steps in Gene...

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Multiple Steps in Gene Expression after Transcription 1. Transcription 2. 5’ Capping 4. Splicing 5. Transport of RNA to Cytoplasm 6. Stabilization/Destabilization of mRNA 7. Translation
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Relative cellular RNA abundance Ribosomal RNAs (rRNAs) ~ 90% Transfer RNAs (tRNAs) ~ 5% Messenger RNAs (mRNAs) ~ 2% The rest (~3%): Signal recognition particle (SRP) RNA Small nuclear RNAs (snRNAs) Small nucleolar RNAs (snoRNAs) Micro RNAs (miRNAs)
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Likely order of events in producing a mature mRNA from a pre-mRNA.
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RNA Capping 1. Post-transcriptional (i.e., G not encoded) 2. Involves adding a 7 Me Guanosine nt to the first (RNA) nt in an unusual way, and often methylation of the first few nt of the RNA. 3. Occurs before the pre-mRNA is 30 nt long
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Fig. 15.3
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Fig. 15.4 AdoMet = S-adenosylmethionine, the methyl donor Capping: order of events and enzymes Product is Cap 1 or “RNA triphosphatase”
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Cap Functions Cap provides: 1. Protection from some ribonucleases 2. Enhanced translation 3. Enhanced transport from nucleus 4. Enhanced splicing of first intron for some pre-mRNAs
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An Unexpected Mechanism for Polyadenylation of pre-mRNA 1. Transcription extends
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Capping & Polyadeynyl - Multiple Steps in Gene...

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