Lec21_RNAEditing - RNA Editing Definition: any process,...

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RNA Editing Definition : any process, other than splicing, that results in a change in the sequence of a RNA transcript such that it differs from the sequence of the DNA template Discovered in trypanosome mitochondria Also common in plant mitochondria Also occurs in a few chloroplast genes of higher plants, and at least a few nuclear genes in mammals K. Stuart L. Simpson
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Discovery of RNA Editing in Trypanosome Mitochondria Unusual Mitos. called Kinetoplasts DNA: Maxicircles (22 kb in T. brucei ), contains most of the genes Minicircles (1-3 kb), heterogenous Sequencing of genomic Mt DNA (Maxicircles) revealed apparent pseudogenes: Full of Stop codons Deletions of important amino acids
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Kinetoplast DNA from a trypanosome visualized by EM Fig. 16.13
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Where were the real functional genes? Investigators generated cDNA clones to some of the kinetoplast mRNAs and sequenced them Sequences were partially complementary to pseudogenes on maxicircle DNA cytochrome oxidase subunit II the COXII DNA sequence above is missing 4 Us found in the mRNA Called this “Editing” because it produced functional mRNAs and proteins from pseudogenes
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COXIII Cytochrome oxidase III From Trypanosoma brucei Fig. 16.15 Lower case Us were inserted by editing.
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This note was uploaded on 08/24/2009 for the course BIO 344 taught by Professor Herrin during the Summer '08 term at University of Texas at Austin.

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Lec21_RNAEditing - RNA Editing Definition: any process,...

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