Final exam 2005 - Bio 344 May 16, 2005 Final Exam Spring...

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Bio 344 Final Exam Spring 2005 May 16, 2005 Name ______________________________________ UTEID _________________________________ Provide the best answer for questions 1-58 on the answer sheet. Answer questions 59 and 60 on the test. There are 160 possible (10 bonus) points. 1. True or False. The major groove of the standard double-stranded DNA molecule is better suited for binding by proteins than the minor groove. a. True b. False 2. Which of the following is the nucleoside 2'-deoxythymidine? 3. The molecule that acts in cells as an enzyme and a carrier of genetic information is a. DNA b. RNA c. proteins d. PNA 4. What is the writhe number (don’t worry about sign) for the DNA shown to the right a. 0 b. > 100 c. 3 d. 4 e. can’t tell 5. True or False. Sliding of the β -clamp is necessary for DNA replication. a. True b. False. 6. Which of the following is/are not true regarding RNA polymerases used for transcription. a. they don’t need a primer b. they don’t need a DNA template c. they polymerize in the 3' to 5' direction d. the reaction catalyzed is the 3' OH of the chain with the terminal phosphate of the incoming NTP e. b-d are not true 7. Which of the following is true regarding the Ac transposon? a. its ends are an inverted repeat b. it encodes a transposase c. it generates a staggered break in the target DNA d. it requires help from Ds to move e. a-c are true 1
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8. Which of the following is required to bind the TATA box? a. TFIIH b. TAF II s c. TBP d. TFIIF e. none of the above 9. The factor that is believed to bind first to Class II promoters, and to be required for binding of the other factors is a. TFIIA b. TFIIB c. TFIIC d. TFIID e. TFIIE 10. Which of the following is a true activation domain in transcription factors a. homeodomain b. glutamine rich sequence c. leucine zipper d. zinc finger e. bHLH domain 11-13. Mix and match the types of recombination and their requirements for sequence homology. 11. Homologous recombination a. no sequence homology 12. Site-specific recombination b. large sequence homology 13. Transposition c. small sequence homology 14. In E. coli transcription, the co-activator for CAP is a. allolactose b. glucose c. cAMP d. lactose 15. In the original classic model of the lac operon, the operator a. is located upstream of the lacZ gene b. binds the lacI gene product c. binds the lac repressor d. all of the above 16. The specificity of DNA binding by the E. coli RNA polymerase is provided mainly by the a. core subunits b. sigma subunit c. beta subunit d. alpha subunit 17. Which of the following is not true regarding the major promoter type in E. coli a. composed of 2 sequence elements upstream of the transcription initiation site
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This note was uploaded on 08/24/2009 for the course BIO 344 taught by Professor Herrin during the Summer '08 term at University of Texas.

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Final exam 2005 - Bio 344 May 16, 2005 Final Exam Spring...

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