ex2_06 - 1Bio 344 -Molecular Biology, Exam II November 2,...

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1 Bio 344 -Molecular Biology, Exam II November 2, 2006 Name_____________________________________ UTEID_________________________________ The computer graded questions (1-41) are worth 2.25 points each; select the best answer and bubble it on the answer sheet. The values of questions 42-46 are indicated, and they should be answered on the test. 1. The DNA in front of the polymerization bubble in the transcribing Thermus aquaticus RNAP is bound by the a. alpha subunit b. beta subunit c. beta’ subunit d. a and b e. b and c 2. The active site of the T. aquaticus RNAP has a a. Zn 2+ b. Mn 2+ c. Mg 2+ d. both a and c e. both b and c 3. The new RNA chain synthesized by the T. aquaticus RNAP a. is initially paired with DNA b. exits out the front channel between 5 and c. exits out a backdoor pore in the sigma subunit d. both a and b e. both a and c 4. Which of the following happens with editing by the E. coli RNAP? a. the RNAP backslides on the template b. the defective part of the RNA sticks out the front of the RNAP c. the Gre factors cleave off the defective part of the RNA d. a and b e. all of the above 5. In the new model of the lac operon, which of the following is not true concerning the operator(s)? a. it is located in 3 places, including two new ones b. they all bind the lacI gene product c. they act cooperatively d. their sequences are identical e. their sequences are similar 6. For the equation lacI +DNAo lacI -DNAo, the ratio [ lacI ][DNAo]/ [ lacI -DNAo] describes a. an equilibrium binding reaction between the lac repressor and the operator DNA b. the Kd for binding c. the rate of dissociation of the lac repressor from the operator DNA d. a and b e. all of the above 1
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7. In the graph below right, which DNA is bound more tightly by the lac repressor? a. O + b. O c c. No operator 8. CAP-cAMP stimulates transcription of the lac operon by a. binding to the activator site b. stimulating formation of the closed complex between the promoter and the RNAP c. stimulating formation of the open complex with the RNAP and promoter d. both a and b e. both a and c 9. Footprinting of a DNA-binding protein with DMS (dimethylsulfate) can reveal
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This note was uploaded on 08/24/2009 for the course BIO 344 taught by Professor Herrin during the Summer '08 term at University of Texas at Austin.

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ex2_06 - 1Bio 344 -Molecular Biology, Exam II November 2,...

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