BIO 126L quiz 1 key

BIO 126L quiz 1 key - wavelength of light is 400 nm Show me...

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BIO126L ( 2-5pm, BME 2.204) TA: Jae Min Lee Date: 06/11/2008 Quiz #1. Name: Hall locker #.: 1-6 Answer True or False (1 point each) 1. 1. The greater the resolution ability of the lens system, the smaller the diameter of the smallest visible structure or object. ( T ) 2. The order of cleaning the objective lenses is 100X, 40X, 10X. ( F ) 3. Parfocal lenses are a set of lenses having focal points lying in the same plane. ( T ) 4. Gram positive bacteria have thick cell walls but Gram negative bacteria have thin cell walls. ( T ) 5. In Gram staining, Gram positive cells are pink and Gram negative cells are purple. ( F ) 6. E.coli is bigger than yeast. ( F ) 7. Oblique transmitted / reflected light microscopy can be used for non-transparent media. ( 1 point ) 8. What are two major types of culture media based on nutritional supply? ( 2 points ) complex chemically defined (synthetic) 9. Determine the resolution when the numerical aperture of objective lens is 1.00 and the
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Unformatted text preview: wavelength of light is 400 nm. Show me how you calculate. Do not forget to write the units properly. ( 4 points ) 400nm/2X1 = 200nm or 0.2um 10. The reason why we use oil for the 100X objective is that the ( refractive index ) of oil is almost same with that of glass. ( two words, 2 points ) 11. 300 nm = ( 0.3 ) μ m (1 point ) 12. List the Gram Staining reagents and briefly define their roles ( 1-2 words maximum ) ( 4 points ) Sol I: 1% crystal violet we can stain the cell walls of both bacteria (gram + and gram -) Sol II: 2% iodine it is mordant. It makes bacterial cell walls hold the crystal violet. Sol III: 95% ethanol destain crystal violet. If you use this long, then even Gram+ bacteria will be destained as Gram – bacteria. Sol IV: 2.5% safranin it counterstains the destained bacterial cells....
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This note was uploaded on 08/24/2009 for the course BIO 126L taught by Professor Saxena during the Summer '08 term at University of Texas.

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