BIO 126L SYLLABUS - SYLLABUS FOR BIO 126 L(Summer 2009 Dr...

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SYLLABUS FOR BIO 126 L (Summer 2009) Dr. Pratibha Saxena You need to be either co-registered in BIO 326R/226N or have taken their equivalent earlier Class website: http://www.sbs.utexas.edu/psaxena/ SCHEDULE OF LAB EXERCISES DATE LAB June 4 Lab check-in Guidelines for success Cleanliness and safety June 9 Lab 1: Use of the light microscope (video-1) June 10 Appendix A: Sterilization Lab 2: Pure culture techniques (video-2, 2 clips) Ubiquity of microorganisms Turn in practice streak plate June 11 Lab 3: Selection and differentiation (requires coming to lab for the next 2 days) Graded Streak Plate (20 points) June 16 Lab 4: Dilution and plating (video-4, 3 clips) Colorimetry and turbidimetry (Graph 10 points) June 17 Lab 5: Identification of organisms (video-5, 5 clips) (requires coming to lab for 4 days) Mixed culture unknown (100 points) June 18 Lab 8: Enrichment culture (takes 10 days but there is some flexibility) June 23 Labs 6: Bacterial growth I (Graph 20 points) (requires coming to lab the next day) June 24 Growth Curve Lab (Graphing) & Review June 25 EXAM I BME 2.106 Labs BUR 108 (Thursday) BME 2.204 Labs BUR 108 2:30p.m. - 4:00p.m. Covers Labs 1-5 June 30 Lab 10: Pathogenic bacteria (requires coming to lab for 4 days) Unknown mixture (120 points) July 1 Lab 9: Phenotypic variation, isolation and (requires coming back on the 2nd and 4th day) characterization of bacterial mutants July 2 Lab 11: Phage and DNA (video-11) (requires coming to lab the next day) (Graph 10 points) July 7 Lab 13: Serology (video - game) Graded Streak Plate (20 points) July 8 Review July 9 EXAM II BME 2.106 Labs BUR 108 (Thursday) BME 2.204 Labs BUR 108 2:30p.m. - 4:00p.m. Covers Labs 6-11 &13 REMEMBER TO CLEAN OUT YOUR INCUBATOR SPACE & HALL LOCKERS BY Thursday July 9 (Worth 20 points of technique/participation) Have your TA check and record that you cleaned out your incubator basket and hall locker
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SUPPLIES AND EQUIPMENT REQUIRED FOR EACH STUDENT 1. Laboratory manual: available at Speedway Printing in Dobie Mall 2. Inoculating wire holder with knurled chuck (wire will be supplied) 3. Pre cleaned microscope slides (2 doz.) 4. Cover slips No. 1 (1 doz.) 5. Combination booklet (lens tissue and bibulous paper) 6. Sharpies 7. Lab coat, chem. apron, or anything to protect your clothes
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