answers - Cell Biology(BIO 320 Summer 2008 Dr Bushart...

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Cell Biology (BIO 320), Summer 2008, Dr. Bushart Supplemental homework for exam #2 1. Based on their localization in an animal cell, what might the signals sequences and structuring of each of these proteins look like? a. Lysosome (functioning there) This would need to start in the ER so it would have a hydrophobic signal. It will have some additional signal to direct it to the lysosome however. Acid hydrolases have M6P tags, so that is one possibility. b. ER resident Needs a hydrophobic sequence, probably cleavable. Soluble ER residents tend to use KDEL signals. c. Integral plasma membrane protein This needs a hydrophobic signal to get to the ER and will then either retain this signal or have more hydrophobic regions. There is no other special signal needed to arrive at the PM 2. You want to engineer a corn plant to produce an insecticidal protein called Grasshopperase. a. (7) You determine that Grasshopperase would be most effective if it was present outside of the cells, in their extracellular matrix. Using what you know about membrane topologies and intracellular transport, which of the following types of signal peptides would you want to have present on the protein: a lysine rich signal, an amphipathic signal, or a hydrophobic signal? Briefly explain (1-2 sentences should be enough!). To exit the cell, the protein needs to start inside the lumen of the ER. Therefore we need a
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This note was uploaded on 08/24/2009 for the course BIO 320 taught by Professor Staff during the Spring '08 term at University of Texas.

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answers - Cell Biology(BIO 320 Summer 2008 Dr Bushart...

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