answers - Cell Biology (BIO 320), Summer 2008, Dr. Bushart...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Cell Biology (BIO 320), Summer 2008, Dr. Bushart Supplemental homework for exam #2 1. Based on their localization in an animal cell, what might the signals sequences and structuring of each of these proteins look like? a. Lysosome (functioning there) This would need to start in the ER so it would have a hydrophobic signal. It will have some additional signal to direct it to the lysosome however. Acid hydrolases have M6P tags, so that is one possibility. b. ER resident Needs a hydrophobic sequence, probably cleavable. Soluble ER residents tend to use KDEL signals. c. Integral plasma membrane protein This needs a hydrophobic signal to get to the ER and will then either retain this signal or have more hydrophobic regions. There is no other special signal needed to arrive at the PM 2. You want to engineer a corn plant to produce an insecticidal protein called Grasshopperase. a. (7) You determine that Grasshopperase would be most effective if it was present outside of the cells, in their extracellular matrix. Using what you know about membrane topologies and intracellular transport, which of the following types of signal peptides would you want to have present on the protein: a lysine rich signal, an amphipathic signal, or a hydrophobic signal? Briefly explain (1-2 sentences should be enough!). To exit the cell, the protein needs to start inside the lumen of the ER. Therefore we need a
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 3

answers - Cell Biology (BIO 320), Summer 2008, Dr. Bushart...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online