bio 320 sample exam 2 - Cell Biology(BIO 320 Spring 2008 Dr Bushart Exam#2 extra questions 1 Based on their localization in an animal cell what might

Cell Biology (BIO 320), Spring 2008, Dr. Bushart Exam #2 extra questions 1. Based on their localization in an animal cell, what might the signals sequences and structuring of each of these proteins look like? a. Lysosome (functioning there) b. ER resident c. Integral plasma membrane protein 2. You want to engineer a corn plant to produce an insecticidal protein called Grasshopperase. a. You determine that Grasshopperase would be most effective if it was present outside of the cells, in their extracellular matrix. Using what you know about membrane topologies and intracellular transport, which of the following types of signal peptides would you want to have present on the protein: a lysine rich signal, an amphipathic signal, or a hydrophobic signal? Briefly explain (1-2 sentences should be enough!). b. After engineering your plants, you find that insects are not affected by eating your corn. The Grasshopperase isolated from the plant is found to be nonfunctional. The amino acid sequence of the isolated enzyme is as