Cell Biology (BIO 320), Spring 2008, Dr. Bushart
Exam #2 extra questions
1.
Based on their localization in an animal cell, what might the signals sequences
and structuring of each of these proteins look like?
a.
Lysosome (functioning there)
b.
ER resident
c.
Integral plasma membrane protein
2.
You want to engineer a corn plant to produce an insecticidal protein called
Grasshopperase.
a.
You determine that Grasshopperase would be most effective if it was
present outside of the cells, in their extracellular matrix. Using what you
know about membrane topologies and intracellular transport, which of the
following types of signal peptides would you want to have present on the
protein: a lysine rich signal, an amphipathic signal, or a hydrophobic
signal?
Briefly
explain (1-2 sentences should be enough!).
b.
After engineering your plants, you find that insects are not affected by
eating your corn. The Grasshopperase isolated from the plant is found to
be nonfunctional. The amino acid sequence of the isolated enzyme is as
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- Spring '08
- Staff
- Animal Cell, Lysosome, Proteins, hydrophobic signal sequence, Grasshopperase
-
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