11 TRANSPIRATION 2009

11 TRANSPIRATION 2009 - Transpiration LAB Phaseolus...

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Transpiration LAB Phaseolus vulgaris L. (Bean plant)
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Objectives Investigate the effect of low light and high light intensity on the rate of transpiration from leaves. Investigate the effect of abscisic acid (ABA) on the rate of transpiration from leaves. Make and view slides of plant root, stem, and leaf sections using bright field and fluorescence microscopy. Examine epidermal peels under the microscope.
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PHOTOSYNTHESIS
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Plant Terms…
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XYLEM Complex vascular tissue through which most of the water and minerals are conducted from the roots to other parts of the plant. Cells are dead.
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XYLEM ELEMENTS * Vessels : Elongated, cells placed end to end. Walls at end of vessel members are perforated or are completly missing. Found in most angiosperms.
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XYLEM ELEMENTS Tracheid : Elongated, thick- walled conducting cells of xylem, characterized by tapering ends and pitted walls without true perforations.
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LIGNIN Hard supporting material embedded in the cellulose matrix of plant cell walls. Makes up xylem inner layer of cell (secondary) walls Second most common plant polymer Autofluoresces blue under UV light
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Phloem Vascular tissue that conducts sugars and other organic molecules from the leaf to the other parts of the plant.
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Sieve tubes Cells of phloem that line up connecting leaves, shoots, and roots. Cells contain little cytoplasm (which is continuous between cells) and no nucleus. Food materials pass from one element to another via sieve plates (perforations).
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PARENCHYMA Plant tissue composed of spongy, living, thin- walled randomly arranged cells with large vacuoles. Usually photosynthetic or storage tissue. Most common type of cell in pants
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A = Xylem B = Phloem Composed of dead cells Cytoplasm of one cell joined to adjacent cells Carries water Carries sugars and organic molecules Wall fluoresce blue under UV light
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Root Morphology Epidermis ( outer tissue absorbs water and minerals from soil) Cortex (ground tissue of root, storage parenchyma) Endodermis (compact cells with no space between them & encircled by continuous band of wax, Casparian strip) Vascular Cylinder (xylem & phloem)
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Jepson, M. (1966). Biological Drawings with Notes , Parts I & II. Norwich:John Murray ( Publishers) Ltd., pp.22-23.
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Vascular Bundle Long continuous strands of conducting tissue in plants. Consist of xylem and phloem tissue close to each other.
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Monocot stem Jepson, M. (1966). Biological Drawings with Notes Publishers) Ltd., pp.22-23.
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Biological Drawings with Notes , Parts I & II. Norwich:John Murray (
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11 TRANSPIRATION 2009 - Transpiration LAB Phaseolus...

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