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Unformatted text preview: early mouse embryos. That the action of only four key transcription factors is sufficient to reprogram adult somatic cells to
pluripotent stem cells is a remarkable finding, which raises a
number of intriguing questions as to the transcriptional programs that control cell fate.
Importantly, further research has also demonstrated that adult
human fibroblasts can be reprogrammed to pluripotency by a
similar procedure. Thus, it is now possible to convert skin cells
from a patient directly to induced pluripotent stem cells in culture, providing a new route to the derivation of pluripotent stem This material cannot be copied, reproduced, manufactured, or disseminated in any
form without express written permission from the publisher.
© 2009 Sinauer Associates, Inc. 27 Culture embryonic
stem cells Differentiate to desired cell type
(e.g., neurons) Transplant back
to patient UNCORRECTED PAGE PROOFS
28 CHAPTER 17 Adult mouse
fibroblast Infection with
genes for Oct3/4,
Sox2, Klf4 and c-...
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