Unformatted text preview: ion from the publisher.
© 2009 Sinauer Associates, Inc. UNCORRECTED PAGE PROOFS
24 CHAPTER 17 KEY EXPERIMENT Culture of Embryonic Stem Cells (continued )
experiment was repeated with additional mouse embryos.
The cell lines derived from normal
embryos (embryonic stem cells) closely
resembled the embryonal carcinoma
cells derived from tumors. Most
importantly, the embryonic stem cells
could be induced to differentiate in
culture into a variety of cell types,
including endodermal cells, cartilage,
and neuron-like cells (see figure).
Moreover, if the embryonic stem cells
were injected into a mouse, they
formed tumors containing multiple
differentiated cell types. It thus
appeared that embryonic stem cell
lines, which retained the ability to differentiate into a wide array of cell
types, could be established in culture
from normal mouse embryos. The Impact
The establishment of embryonic stem
cell lines has had a major impact on
studies of mouse genetics and development as well as opening new...
View Full Document