Cooper5e_Ch17

Her experiments together with similar work by martin

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Unformatted text preview: mbryos, Nature, 1981, 292: 154–156), demonstrated that stem cells could be cultured directly from (C) Gail R. Martin normal mouse embryos. The isolation of these embryonic stem cell lines paved the way to genetic manipulation and analysis of mouse development, as well as to the possible use of human embryonic stem cells in transplantation therapy. The Experiments Based on the premise that embryonal carcinoma cells were derived from normal embryonic stem cells, Martin attempted to culture cells from normal mouse blastocysts. Starting with cells from approximately 30 embryos, she initially isolated four colonies of growing cells after a week of culture. These cells could be repeatedly passaged into mass cultures, and new cell lines could be reproducibly derived when the Embryonic stem cells differentiate in culture to a variety of cell types, including neuronlike cells (A), endodermal cells (B), and cartilage (C). (Continued on next page.) This material cannot be copied, reproduced, manufactured, or disseminated in any form without express written permiss...
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