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Unformatted text preview: Myc Induced
stem cell FIGURE 17.27 Induced pluripotent stem cells Adult mouse fibroblasts in culture are converted to pluripotent stem cells by infection with retroviral vectors (see
Figure 4.34) carrying genes for four transcription factors: Oct3/4, Sox2, Klf4, and
c-Myc. cells for use in transplantation therapy. However, problems still remain to
be addressed. Some of the transcription factors (e.g., c-Myc) used to reprogram fibroblasts to pluripotent stem cells can act as oncogenes to cause cancer, although it may be possible to substitute these potentially harmful transcription factors with others that do not have oncogenic potential.
Additionally, the retroviral vectors that have been used to introduce genes
into fibroblasts can themselves cause harmful mutations leading to cancer
development, so alternative methods of gene transfer will need to be developed for safe therapeutic applications. Nonetheless, overcoming these difficulties may enable induced pluripotent stem cells to be readily established
and used for patient-specific transplantation therapy. SUMMARY KEY TE...
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