315 Homework #4

315 Homework #4 - MET VAL HIS LYS ARG THR LEU VAL After...

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BIO 315 University of Kentucky Dr. E. Rucker III Assignment 4 Terpsichore Lindeman St. No. 10668240
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1) About 30% of point mutation (substitutions) that occur in coding regions of DNA are harmless. Why? (1 pt) c) Because most amino acids are specified by several codons that differ at the third position 2) Which type of mutation is likely to be the most harmful? (1 pt) b) A +3 frame shift mutation 3) Niremberg used the enzyme polynucleotide phosphorylase to assemble RNAs with random sequences in the absence of a template. Suppose they added the enzyme to a solution of 90% CTP and 10% UTP, and then used a cell free system of protein synthesis to make peptides according the sequences in their synthetic RNAs. (4 pts) a) Which amino acids could possible appear in the synthetic protein? PHE, LEU, SER, PRO b) Which amino acid would be the most abundant in the synthetic protein? PHE c) Which amino acid would be least abundant? PRO 4) A bacterium produces a normal protein with the following amino acid sequence:
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Unformatted text preview: MET VAL HIS LYS ARG THR LEU VAL After irradiation, a mutant strain is produced that makes a mutant protein from the same coding site on DNA: MET VAL HIS LYS GLU PRO Describe the mutation that occurred ( 4 pts) 5) What is a nuclear localization signal, and what importance does it serve? (1 pt) A nuclear localization signal is a specific signal for transported ribosomal proteins that are created a lot. It comes with its own importin nuclear receptors. Basically it attracts a protein that it wants to import to the cell nucleus. It is a sequence of amino acids that lays on the surface of a protein and kind of tags itself. The amino acid sequence it has is to be used when it has met with the target protein to take the protein to the cell nucleus through the Nuclear Pore Complex. Its importin receptors are signals for the nuclear transport receptors of protein that is newly synthesized....
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This note was uploaded on 08/25/2009 for the course BIO 315 taught by Professor Steiner during the Spring '08 term at Kentucky.

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315 Homework #4 - MET VAL HIS LYS ARG THR LEU VAL After...

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