BIO 315assignment 1

BIO 315assignment 1 - ST Number 10668240 BIO 315 University...

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ST Number 10668240 BIO 315 University of Kentucky Dr. E. Rucker III Terpsichore Lindeman ST Number 10668240
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[ ASSIGNMENT 1 ]
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ST Number 10668240 Contents 1) Give the significance of the following terms. Say what they do  or why they are important.  (1 pt each) a) Microscopic resolution In order to fully appreciate what importance microscopic resolution has we must understand the difference between magnification and resolution. Microscopic resolution is the level of ability that a microscope has to help us optically distinguish detail of closely adjacent objects. This is of grave importance when examining anything under a microscope as distances between objects in the micro-cosmos are minimal. b) Electron microscopy Electron Microscopy (EM) is the most essential type of microscopy for any cytobiologist. When EM is used properly it assists in providing a detailed image of cells on a molecular level revealing the organization of the cell in view and subcellular structures. c) Two dimensional gel electrophoresis Two-dimensional gel electrophoresis is mainly used in order to be able to identify proteins as individual molecules. As we know most proteins in cells come together and become a new “entity” to function and thus in order to identify each protein molecule on its own we must “separate them”. In order to do so we use this method as we all know that different molecules react differently when their PH environment is altered. Thus they attain polarization and can “sway” to the opposite polar along a long length to be identified. Such a method is crucial not just for scientific research but for examining food. In November of 2008 when the scandal broke out of tainted milk, milk with melamine- it was distinguished that usual tests only tested products for the level of protein which melamine when “mixed” with watered down milk enhances. Via the two dimensional gel electrophoresis process, investigators were able to identify the other “pseudo protein” melamine. d) Polymerase chain reaction
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This note was uploaded on 08/25/2009 for the course BIO 315 taught by Professor Steiner during the Spring '08 term at Kentucky.

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BIO 315assignment 1 - ST Number 10668240 BIO 315 University...

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