StructureandFunctionofImmunoglobulins

- BIO/MI494G Structure and Function of Immunoglobulins Fall 2008 I II Definition of immunoglobulin and antibody General characteristics of

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
BIO/MI494G Structure and Function of Immunoglobulins Fall 2008 I. Definition of immunoglobulin and antibody II. General characteristics of immunoglobulins 1. Historical perspective a. gamma globulin 2. Forms of immunoglobulins plasma membrane receptors on B-cells b. secreted by plasma cells 3. Function of immunoglobulins specifically bind free antigen or cell associated antigen participate in general effector functions 4. Immunoglobulins are divalent or multivalent 5. Basic structure of immunoglobulins light (L) chains heavy (H) chains 6. Classes of immunoglobulins five classes: IgG, IgM, IgA, IgE, IgD heavy chains: gamma, mu, alpha, epsilon and delta c. light chains: kappa and lambda III. Immunoglobulin structure in more detail Intra and inter molecular disulfide bonds Fab and Fc fragments - proteolytic cleavage by papain F(ab'h and Fc fragments - proteolytic cleavage by pepsin 4. Constant and variable regions 5. Hypervariable (complementarity - determining regions) and framework regions IV. IgG General properties Physiochemical properties 3. Structure 4. Biologic function V. IgM General Properties Physiochemical properties Structure IgM subunits J-chain Biologic function
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
VI. IgA 1. 2. 3. 4. VII. IgE 1. 2. 3. VIII. IgO 3. General properties Physiochemical properties of serum IgA Physiochemical properties of secretory IgA a. dimeric and trimeric forms b. J-chain c. acquisition of secretory piece from epithelial cells Functional properties of serum and s.ecretory IgA General properties Physiochemical properties Biologic function General Properties Physiochemical properties Biologic function IX. Antigenic determinants on immunoglobulins Isotypes Allotypes 3. Idiotypes
Background image of page 2
Feature I Antigen bindingmofecule I -.- -'--' T .. - MHC molecules* : . _ .•--- _ •.•-,.-. .#. . . -_. __. ..• --- ~ .. -----+.--,-.- . ._. --.-. Antigen ; Made up of three I Made up of three Peptide-binding deft ! CDRs in VH and i CDRs in Va and made of a 1and a2 (class binding site j three CDRs in VL I three CDRs in Vo or (Il and ~ 1 (class II) . _~ .. --"._-.__ ._-._- i-····-· --- - -- _. ... .- .-- i--------- - --- Nature of antigen I Macromolecules (proteins, i Peptide-MHC Peptides that may be bound j lipids, polysaccharides) i complexes . 1 and small chemicals i I -~--. ..j . . . . . ····-------I-~-··-----·-··-----·- ----- r-----·· ----.--. . ----.~.-. Nature antigenic 1 Unear and conformational I linear determinants of I Linear determinants of determinants 1 determinants ofvarious I peptides; only 2or 3 amino i peptides; only some recognized ! macromolecules acid residues of a peptide t amino acid residues i and chemicals ,i bound to an MHC molecule ! of a peptide i ----. . -------~_.-._-- -1 . . --- ._-:- -..-_ -- "'---'--'-"--'-"'-~"r'--"-'~'---'--"-'--'-"-'-" . _- '--' --. . _-- __.__ L.... __. ..•. .--- --.---.------ Affinityof ! K;J10- 7 -10· l1 M;average i ~Jl05_1O-7M I K;11O-o-1O- 9 M; antigen binding ! affinity of Ig5 increases extremely stable binding I If i during immune response I I ___. ._._. . ._. ._ . _ •._.1. __ . .•, ..•_. ..J. ... __. .__ __.•••. .__ ---'-. . . . _ ._ . ._ "The structures and functions of MHC and TCR molecules <Ire discussed in Ch3ptefS 5 (Ind 7. respectively_ Abbreviations: CDR, complem€ntarity-determining region; I<d, dissociation constant; MHC, major histocompatibility complex; V H variable domain of heavy chain Ig; V l , variable domain oflight chain 19 Copyn:;nl C;, 21)0.7 b, Saunoeffi. an ImPrInt "f EIS';>'/Ier Inc.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
i .
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

This document was uploaded on 08/25/2009.

Page1 / 16

- BIO/MI494G Structure and Function of Immunoglobulins Fall 2008 I II Definition of immunoglobulin and antibody General characteristics of

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online