Biology Chapter 20 Objectives

Biology Chapter 20 Objectives - Chapter 20 DNA Technology...

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Chapter 20 DNA Technology and Genomics DNA Cloning 2. The natural function of restrictions is to protect the bacterial cell against intruding DNA from other organisms, such as phages or other species of bacteria. They are used in recombinant DNA technology to recognize a particular short DNA sequence, or restriction site, and cut both DNA strands at specific points within this restriction site. 3. The creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA molecule because these short extensions can form hydrogen- bonded base pairs with complementary stick ends on any other DNA molecules cut with the same enzyme. 4. In order to clone a eukaryotic gene in a bacterial plasmid, first we need to isolate the bacterial plasmid and DNA containing the gene of interest. Then, both the plasmid and the human DNA are digested with the same restriction enzyme, one that produces sticky ends; the enzyme cuts the plasmid DNA at its single restriction site. Then, mix the cut plasmids and DNA fragments. Some will join by base pairing but DNA ligase is needed to seal them together. The DNA is then introduced into bacterial cells, and some cells will take up a recombinant plasmid or other DNA molecule by transformation. Finally, plate the bacteria and incubate until colonies crow. 5.
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Biology Chapter 20 Objectives - Chapter 20 DNA Technology...

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