Aseptic Technique - Aseptic Technique Running head: ASEPTIC...

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Aseptic Technique 1 Running head: ASEPTIC TECHNIQUE Aseptic Technique Jennifer Herr Chamberlain College of Nursing (Devry)
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Introduction: A 4-year old girl was brought to the emergency room suffering from severe, watery diarrhea, lethargy, and nosebleeds. Emergency room personnel reported a fever of 101 o F, blood pressure of 110/70, and heart rate of 72. Blood and stool samples were taken. Urine samples contained blood. The blood work revealed an acidic pH and abnormally low levels of platelets and erythrocytes. High levels of creatinine and urea were observed. This organism was cultured from various body fluids. The purpose of this lab to see if the microbe can ferment the carbohydrate glucose as a carbon source. Bacteria are identified based largely on what organic compounds they can break down. The range of compounds used depends on the collection of enzymes a species of bacteria can make. Glucose is a sugar that some bacteria can use because of an enzyme that begins the breakdown of this compound. Phenol red glucose (dextrose) broth is thus used to determine whether the microbe can use the sugar glucose for carbon and energy. If glucose is fermented to produce acid end products, the pH of the medium will drop therefore a pH indicator in the medium changes color to indicate acid production. In this experiment I used is phenol red glucose (dextrose) broth. The medium is a nutrient broth to which 0.5-1.0% glucose is added. The pH indicator phenol red is red at neutral pH but turns yellow at pH <6.8. It also changes to magenta or hot pink at pH >8.4. Additionally, this medium contains a Durham tube, a smaller inverted tube which can serve as a trap for gas bubbles generated during fermentation of sugars. An inoculum from a pure culture is transferred aseptically to a sterile tube of phenol red glucose
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This note was uploaded on 09/02/2009 for the course BIO 111 taught by Professor Smith during the Spring '09 term at Columbus State University.

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Aseptic Technique - Aseptic Technique Running head: ASEPTIC...

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