exam 2 - BL/CH401 EXAM 2 October 27th 1999 1 50 Pts Enzyme...

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BL/CH401 EXAM 2 October 27 th 1999 1. 50 Pts. Enzyme Kinetics Problem Draw plots of Vo vs [S] and 1/Vo vs 1/[S], using the data given below - 25 Pts Determine Km and Vmax for uninhibited reaction (include units) - 10 Pts Determine what type of Inhibitor A and B are using the Km’ and Vmax’ for the inhibitors (show your reasoning by comparing Vmax and Vmax’ and Km and Km’) - 10 Pts Calculate the Ki for binding of Inhibitor A and B to the enzyme (include units) - 5 Pts [S] Vo (No I) Vo (I = 0.1 mM A) Vo (I = 2.0 mM B) mM μmol/min μmol/min μmol/min 0.0 0.0 0.0 0.0 0.2 20.0 8.0 9.1 0.4 33.3 13.3 16.7 1.0 55.6 22.2 33.3 2.0 71.4 28.6 50.0 10.0 92.6 37.0 83.3 For Competitive Inhibitor, Km' = Km (1 + [I]/Ki) For Non-Competitive Inhibitor, Vmax' = Vmax / (1 + [I]/Ki) Vo vs [S] Plot 0 10 20 30 40 50 60 70 80 90 100 0 2 4 6 8 10 [S], mM Vo , μmol/min No Inh I = A I = B ~Vmax = 100 ~Km = 1 mM
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Double Reciprocal Plot y = 0.02x + 0.025 R 2 = 1 y = 0.02x + 0.01 R 2 = 1 y = 0.008x + 0.01
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This note was uploaded on 09/02/2009 for the course BIO BL 401 taught by Professor Wilbur during the Spring '07 term at Michigan Technological University.

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exam 2 - BL/CH401 EXAM 2 October 27th 1999 1 50 Pts Enzyme...

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