lec 12 - BL/CH401 Lecture 12 Enzyme Kinetics Part I I...

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BL/CH401 Lecture 12 -- Enzyme Kinetics -- Part I I. Introduction to Enzyme Kinetics. In chemistry, kinetics has to do with the rate of reactions. In biochemistry, we are most interested in rates of enzyme catalyzed reactions since virtually all biological reactions are catalyzed by enzymes. Enzyme Kinetics: Rates of enzyme catalyzed reactions Usefulness of enzyme kinetics: Common clinical assays to detect enzymes Understanding metabolic pathways Measuring binding of substrates and inhibitors to the active site of an enzyme Understanding the mechanism of catalysis of an enzyme Rates of reactions are measured by change in reactant amounts with time. You can measure the disappearance of the substrate or the appearance of the product. Usually, the appearance of the product is easier to keep track of since there should be no product present at the beginning of the reaction. Figure 1. Ways to express a rate for the enzyme catalyzed reaction. Rates = Reaction Velocity For enzymes, the initial velocity (before significant product accumulates) is always used. Initial Velocity = Vo Part II. A Simple Mechanism for the Enzyme Catalyzed Reaction. For catalysis to begin, the substrate must bind to the enzyme, which results in the formation of the enzyme-substrate complex (ie E-S complex). The E-S complex forms rapidly in the first part of the enzyme catalysis process and the concentration of the E-S stays constant at a steady-state
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level. For this reason, this type of kinetics is called steady-state kinetics . A simple mechanism for the enzyme catalyzed reaction helps us to understand and model this process. Figure 2. A simple enzyme mechanism for a single substrate and product. This simple mechanism can also be expressed with rates shown for each process in the mechanism: Figure 2a. Simple enzyme mechanism with rate constants shown for each step. Part III. Enzyme Catalyzed Rates at Different Substrate Concentrations.
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lec 12 - BL/CH401 Lecture 12 Enzyme Kinetics Part I I...

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