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problems - BL401/CH401 Biochemistry-AMINO ACID(AA SEQUENCE...

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BL401/CH401 -- Biochemistry --AMINO ACID (AA) SEQUENCE PROBLEMS 1. Acid hydrolysis and AA analysis of a peptide 1 yielded: Arg, Glu, 2 Val, Gly, Lys, Tyr, Thr & Phe. A) Dansylation gave Dansyl-Glu, while Thr was released first by carboxypeptidase. B) Cleavage of peptide 1 with trypsin gave 3 peptides: T-1, T-2 & T-3. T-1 was a tripeptide; T-2 a dipeptide; T-3 a tetrapeptide. T-1 had AA composition = Arg, Tyr, Glu. N-terminal of T-2 was Val. Edman degradation of T-3 gave Phe, while its C-terminal was Thr. C) Cleavage of peptide 1 with chymotrypsin gave 3 peptides: C-1, C-2 & C-3. C-1 was a tripeptide; C-2 a dipeptide; C-3 a tetrapeptide. N-terminal of C-1 was Gly, while its C-terminal was Thr. AA composition of C-2 was Tyr, Glu. N-terminal of C-3 was Arg. What is the AA sequence of peptide 1? Draw the structure of peptide 1 at pH 7. What is the net charge of peptide 1 at pH 7 What is its pI? 2. After cleavage of a protein X with cyanogen bromide (CNBr), peptide 2 was isolated and it was not the C- terminal peptide. A) Treatment of peptide 2 with trypsin proved negative.
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