problems 2 answers - BL/CH401 - Enzyme Kinetics Problems...

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BL/CH401 -- Enzyme Kinetics Problems Answers Problem 1 Figure 1. V vs. [S] plot
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Figure 2. How to Establish Limits for the X and Y Axes on 1/Vo vs. 1[S] plot. Figure 3. 1/Vo vs. 1[S] plot. Information taken from 1/Vo vs. 1/[S] graph: -1/Km = 0.56 so Km = 1.8 mM 1/Vmax = 0.073 so Vmax = 14 micromol/min Don't Forget the Units for Km and Vmax Also BE SURE to USE Correct number of Significant Figures!!
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Check the Vmax and Km you found using the M-M equation: Figure 4. Michaelis-Menten Equation. Do check by plugging in Km = 1.8 mM and Vmax = 14 micromol/min; What is Vo at [S] = 1 mM? Vo = (14) x (1) / (1.8 + 1) = 5 micromol/min Your answer in the check should be +/- 10% of the value given in the data set. Problem 2 Figure 5. V vs. [S] plot
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Figure 6. How to Establish Limits for the X and Y Axes on 1/Vo vs. 1[S] plot. Figure 7. 1/Vo vs. 1[S] plot.
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Information obtained from 1/Vo vs. 1[S] graph: Table of Results for Kinetic Constants: Reaction Vmax or Vmax' Km or Km' Type Inhibitor (units) nmol/min
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problems 2 answers - BL/CH401 - Enzyme Kinetics Problems...

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