lecture14

lecture14 - Lecture 14 Lecture 14 Lecture 14 Lecture 14...

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Unformatted text preview: Lecture 14 Lecture 14 Lecture 14 Lecture 14 Lecture 14 Gene Complementation in Bacteria Gene Complementation in Bacteria Gene Complementation in Bacteria Gene Complementation in Bacteria Gene Complementation in Bacteria In order to perform tests for dominance or for complementation in bacteria we need a way to make the bacteria diploid for part of the chromosome. To do this we need to consider a different extrachromosomal element: Ori T The F plasmid (length 10 5 base pairs) Tra genes There are some special terms to describe the state of F in a cell: F refers to a strain without any form of F, F, F, F, F, whereas F + refers to a strain with an F plasmid. Donor cell Recipient cell Ori T F pilus F is very efficient at transferring itself from an F + cell to an F cell. After culturing F + and F cells together about 1/10 of the F cells will become F + . The property that makes F useful for genetic manipulation is that at low frequency the plasmid will integrate into chromosome. This occurs because plasmid will integrate into chromosome....
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This note was uploaded on 09/03/2009 for the course BIOL 7.03 taught by Professor Chriskaiser during the Fall '04 term at MIT.

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lecture14 - Lecture 14 Lecture 14 Lecture 14 Lecture 14...

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