MCDB428Lect13 - MCDB 428 Lecture 13: Wednesday February 6,...

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Lecture 13: Wednesday February 6, 2008 Today’s Outline Overview of Vesicle Trafficking Pathways Biosynthetic secretory pathway Retrograde trafficking and retrieval pathways Endocytic trafficking pathways Three main types of coated vesicles COPI coated vesicles COPII coated vesicles Clathrin coated vesicles Sorting signals that are recruited to these different vesicles The role of GTPases in vesicle budding and fusion Basic GTPase cycle Role of GEFs and GAPs in activation/inhibition of GTP-proteins COPII vesicle formation Sar1 activation Inner coat recruitment Outer coat recruitment and release COPI coated vesicles role in membrane trafficking Retrograde trafficking vs. anterograde trafficking Formation of the cis-Golgi network Retrieval of ER-resident proteins from the Golgi KDEL-receptor cycle COPI vesicle formation Arf1 GTPase COPI coatomer recruitment The role of ArfGAP Brefeldin A Effect on membrane trafficking Mechanism of action MCDB 428
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Road Map of the Secretory and Endocytic Pathways Red arrows indicate biosynthetic secretory pathways involved in trafficking and sorting of newly synthesized cargo Blue arrows indicate retrograde trafficking pathways involved in retrieval of cargo and sorting receptors Green arrows indicate endocytic trafficking pathways involved in uptake of extracellular cargo
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Three main types of of vesicular carriers in Eukaryotic cells 1). COPI vesicles: intra-Golgi and retrograde Golgi-ER transport 2). COPII vesicles: ER-Golgi anterograde protein transport 3). Clathrin vesicles: TGN-PM, TGN-Lysosome, PM-endosome
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Use of Different Coats in Vesicular Traffic COPI coats used to form transport vesicles involved in anterograde transport from ER to Golgi COPII coats used in retrograde transport between Golgi cisternae and from Golgi to ER Clathrin coats used in post-Golgi secretory and endocytic membrane trafficking steps
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Transport Vesicles Have Distinct Coats Different transport steps use different coat proteins to help sort and concentrate cargo
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MCDB428Lect13 - MCDB 428 Lecture 13: Wednesday February 6,...

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