MCDB428lect17 - MCDB 428 Lecture 17: Friday February 15,...

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Lecture 17: Friday February 15, 2008 Today’s Outline Polar Growth in Budding Yeast Cell replication in budding yeast Schmoo formation during mating Bud site selection Recruitment of the exocyst Sec4 vesicle targeting The Neuromuscular junction (NMJ) Description of the NMJ Basal lamina, what it is Role of basal lamina in NMJ formation AChR receptor clustering Agrin, MuSK and Rapsyn function in AChR clustering Establishment of Polarized Plasma Membrane Domains Apical and Basolateral PM domains in different cell types Organization of epithelial sheets in vitro Role of Cell—Cell contacts in establishing tight junctions Sustaining polarization through continuous membrane trafficking Apical and basolateral targeting signals Experimental investigation of apical and basolateral trafficking pathways MCDB 428
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Cell Polarity: Effects of polarization on Membrane Trafficking, but how is Polarity established and maintained? Today we’ll talk about 3 different polarized cell systems and Discuss mechanisms by which polarity is generated in different Types of eukaryotic cells
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Polarized Growth In Budding Yeast In budding yeast cell division occurs via the formation of a daughter “bud” at late stages of the G1 stage of the cell cycle During progression through the G1 and S phases of the cell cycle cell components are selectively trafficked to this daughter bud allowing it to grow in size Eventually, once DNA replication has occurred, one copy of the genome will be transferred to the daughter bud and cytokinesis occurs resulting in formation of two cells
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Polarization of S. cerevisiae does not only occur during cell division Under nutrient limiting conditions yeast can sporulate, but only as diploids (normally they live as haploids) Cell fusion only occurs between different mating types (“a” and “ α ”) Because
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This note was uploaded on 09/03/2009 for the course MCDB 428 taught by Professor Wang during the Winter '08 term at University of Michigan.

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MCDB428lect17 - MCDB 428 Lecture 17: Friday February 15,...

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