Final review - resistant cyclin protein and how would they...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
In the Wnt signaling pathway, the Wnt ligand binds to a receptor complex on the cell surface. This interaction leads to the inhibition of a protein kinase GSK. In the absence of Wnt signal, GSK phosphorylates β -catenin, which causes β -catenin to become ubiquitinated and degraded. In the presence of Wnt signaling, β -catenin accumulates and moves to the nucleus, where it binds to TCF, a DNA-binding protein that binds Wnt target gene promoters. The binding of β -catenin to TCF converts TCF from a transcriptional repressor to an activator. 1) Design a dominant negative version of TCF, which can block the ability of Wnt to activate target genes. 2) Design a β -catenin mutant that is constitutively active (i.e. activates Wnt target genes in the absence of Wnt signal).
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
A. Describe the classic experiment proving that cyclin  degradation is required to complete mitosis and continue with the  cell cycle.
Background image of page 2
B. What kind of mutation in cyclin B might produce a degradation-
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
Background image of page 5
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: resistant cyclin protein and how would they cause this property? 1. Chromosome DNA present in diffuse,extended form during 2. Mitotic cyclin is at its lowest level in 3. A Cdk (cdc2) protein is present in cell during 4. p53 is activated if DNA is damaged 5. Nuclear lamin is phosphorylated 6. Pre-RC (pre-replicative complex) is assembled 7. The ubiquitin ligase APC is activated 8. p21CIP over expression blocks cell at 9. When cells are treated with nocodazole for 22 hours (suppose they do not die) 10. Centriole starts to replicate Study the statements in the column on the left, and mark the appropriate column(s) on the right to indicate the phase of the cell cycle for which the statement is true. G1 S G2 M Several laboratories are trying to identify small, membrane-permeable molecules that can inhibit IAP family members. They hope such molecules will help in the treatment of some cancers. Please explain why IAP is a potential target for anti-cancer treatment?...
View Full Document

This note was uploaded on 09/03/2009 for the course MCDB 428 taught by Professor Wang during the Winter '08 term at University of Michigan.

Page1 / 5

Final review - resistant cyclin protein and how would they...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online